Composite guidance scaffolds for neural tissue engineering

No abstract available

Inhibitory effects of soluble fibronectin fractions on cultured neurones

No abstract available

Increased GFAP immunoreactivity by astrocytes in response to contact with dorsal root ganglia cells in a 3D culture model

Failure of repair mechanisms in the injured CNS is widely attributed to the inhibitory environment of the lesion site, most notably the formation of the glial scar which forms a physical and physiological barrier to axon regeneration. We developed an in vitro 3D cell culture model to investigate the response of astrocytes to cells found at the inhibitory interfaces formed following damage to the spinal cord. CellTrackerTM labelled dissociated DRGs were seeded onto astrocyte-populated collagen gels and maintained in culture for 5 days. Astrocytes near the DRG interface showed marked GFAP up-regulation and adopted a reactive morphology which was observed up to 1mm away. Intensity of GFAP fluorescence at this interface was 3 fold higher than that seen away from the interface or in controls (astrocyte only gels). Furthermore, the presence of DRG conditioned medium was not capable alone of eliciting this response. In conclusion this model may provide a useful tool for understanding reactive astrogliosis in response to cells found at inhibitory interfaces following spinal cord or dorsal root injury. The contact between astrocytes and satellite cells may be enough to induce astrocyte reactivity and formation of the gliotic scar, or this contact may induce the secretion of a soluble factor which is not released from DRG cultures under physiological conditions

Lifeguard: Local Health Awareness for More Accurate Failure Detection

SWIM is a peer-to-peer group membership protocol with attractive scaling and robustness properties. However, slow message processing can cause SWIM to mark healthy members as failed (so called false positive failure detection), despite inclusion of a mechanism to avoid this. We identify the properties of SWIM that lead to the problem, and propose Lifeguard, a set of extensions to SWIM which consider that the local failure detector module may be at fault, via the concept of local health. We evaluate this approach in a precisely controlled environment and validate it in a real-world scenario, showing that it drastically reduces the rate of false positives. The false positive rate and detection time for true failures can be reduced simultaneously, compared to the baseline levels of SWIM

Abnormal attentions towards the British Royal Family. Factors associated with approach and escalation

Abnormal approach and escalation from communication to physical intrusion are central concerns in managing risk to prominent people. This study was a retrospective analysis of police files of those who have shown abnormal attentions toward the British Royal Family. Approach (n = 222), compared with communication only (n = 53), was significantly associated with specific factors, most notably serious mental illness and grandiosity. In a sample of those who engaged in abnormal communication (n = 132), those who approached (n = 79) were significantly more likely to evidence mental illness and grandiosity, to use multiple communications, to employ multiple means of communication, and to be driven by motivations that concerned a personal entitlement to the prominent individual. Logistic regression produced a model comprising grandiosity, multiple communications, and multiple means of communication, for which receiver operating characteristic (ROC) analysis gave an area under the curve (AUC) of 0.82. The implications of these findings are discussed in relation to those for other target groups

Forward Exchange Market Unbiasedness: The Case of the Australian Dollar Since 1984

This paper implements a new statistical approach to robust regression with nonstationary time series. The methods are presently under theoretical development in other work, and are briefly exposited here. They allow us to perform regressions in levels with nonstationary time series data, they accommodate data distributions with heavy tails and they permit serial dependence and temporal heterogeneity of unknown form in the equation errors. With these features the methods are well suited to applications with frequently sampled exchange rate data, which generally display all of these empirical characteristics. Our application is to daily data on spot and forward exchange rates between the Australian and US dollars over the period 1984-1991 following the deregulation of the Australian foreign exchange market. We find big differences between the robust and the non-robust regression outcomes and in the associated statistical tests of the hypothesis that the forward rate is an unbiased predictor of the future spot rate. The robust regression tests reject the unbiasedness hypothesis but still give the forward rate an important role as a predictor of the future spot rate.

Force transmission across a core-sheath interface in peripheral nerves [poster presentation]

No abstract - poster presentation